![]() 1 Their regulatory impact on allogeneic immune reactions, such as graft-versus-host disease (GVHD), occurring after bone marrow transplantation makes MSCs an attractive tool of low toxicity for cell-based therapies. Nowadays, it is well established that human MSCs possess a plethora of immunomodulatory properties. Mesenchymal stem cells (MSCs) are a heterogeneous population of fibroblast-like, multipotent cells characterized by their ability to differentiate in vitro and in vivo into tissues of the mesodermal lineage. Taken together, we show that HO-1 produced by human MSCs beyond its direct suppressive function promotes formation of Tr1 and Th3 Tregs and IL-10 production, functions, which are taken over by other molecules, among them COX-2, after an alloreactive priming. Interestingly, HO-1 lost its impact regarding suppressiveness, Treg induction, and promotion of IL-10 production for MSCs, which were prelicensed in an MLR environment. We observed a substantial down-regulation of HO-1 facilitated by yet unidentified soluble factor(s) produced in an MLR, and most probably occurring at the level of its major transcription-factor NF-E2–related factor 2. Because inflammatory stimuli modulate (“license”) human MSCs, we were interested in whether an in vitro alloreactive micro-milieu within mixed lymphocyte reactions (MLRs) alters the HO-1 expression. Human MSCs were shown to induce, in a HO-1–dependent fashion, IL-10 + Tr1 and transforming growth factor-β + Th3 Treg-subsets in allo- and T-cell receptor-activated lymphocytes. As HO-1 has also been implicated in the induction of regulatory T cells (Tregs), we sought to examine its impact on MSC-driven promotion of Tregs. The stress-responsive, cytoprotective, and immunoregulatory molecule heme oxygenase-1 (HO-1) was recently identified as a key contributor for MSC-mediated suppression of alloactivated T cells. This anonymized visitor and browsing information is stored in Google Analytics.Īny changes we may make to our Cookie Policy in the future will be posted on this page.Mesenchymal stem cells (MSCs) are characterized by their manifold immunomodulatory and regenerative properties. The cookies collect information in an anonymous form, including the number of visitors to the website, where visitors have come to the site from and the pages they visited. We use the information to compile reports and to help us improve the website. _ga_UA-#: These cookies are used to collect information about how visitors use our site.cookieconsent_status: This cookie is used to remember if you've already dismissed the cookie consent notice.locale: This cookie is used to remember your locale and language settings.registrarToken: This cookie is used to remember items that you have added to your shopping cart.JSESSIONID: This cookie is used by the application server to identify a unique user's session. FLOWJO DEPARTMENTAL LICENSE UPDATEWe may update the information contained in this section from time to time. The list below identify the cookies we use and explain the purposes for which they are used. ![]() FLOWJO DEPARTMENTAL LICENSE HOW TOTo find out more about cookies, including how to see what cookies have been set and how to manage and delete them please visit. Most web browsers allow some control of most cookies through the browser settings. The information these cookies collect may be anonymized and they cannot track your browsing activity on other websites. For instance, a functional cookie can be used to remember the items that you have placed in your shopping cart.
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